Prepare fasta sequence database for re-pcr searches
famap [-hV] -b mmapped-file [-t cvt] [fafile ...]
famap [-hV] -d mmapped-file [ord ...]
famap [-hV] -l mmapped-file [ord ...]
The program famap is part of the e-PCR suite and is used to build mmapped-file from FASTA files for reverse e-PCR searches
where cvt (conversion table) is one of:
off - as is (default)
n - nucleotide [acgtnACGTN] allowed,
N - nucleotide uppercase allowed [ACGTN]
nx - nucleotide with ambiguity codes allowed
NX - nucleotide with ambiguity codes uppercase
famap -tN -b genome.famap org/chr_*.fa
fahash -b genome.hash -w 12 -f3 ${PWD}/genome.famap
re-PCR -s genome.hash -n1 -g1 ACTATTGATGATGA AGGTAGATGTTTTT 120-200
See famap(1) and re-pcr(1)
/usr/share/doc/ncbi-epcr/README.txt
bioperl(1), e-pcr(1), famap(1) and re-pcr(1)
This manual page was written by Andreas Tille <[email protected]> for the Debian system (but may be used by others). Permission is granted to copy, distribute and/or modify this document under the terms of the GNU General Public License, Version 2 any later version published by the Free Software Foundation.
On Debian systems, the complete text of the GNU General Public License can be found in /usr/share/common-licenses/GPL.