last-pair-probs(1)

last-pair-probs1

Read alignments of paired dna reads to a genome, and do estimations

Usage

last-pair-probs [options] interleaved-alignments

last-pair-probs [options] alignments1 alignments2

Overview

Read alignments of paired DNA reads to a genome, and: (1) estimate the distribution of distances between paired reads, (2) estimate the probability that each alignment represents the genomic source of the read.

Options

-h, --help

show this help message and exit

-r, --rna

assume the reads are from potentially-spliced RNA

-e, --estdist

just estimate the distribution of distances

-m M, --mismap=M

don't write alignments with mismap probability > M (default: 0.01)

-f BP, --fraglen=BP

mean distance in bp

-s BP, --sdev=BP

standard deviation of distance

-d PROB, --disjoint=PROB

prior probability of disjoint mapping (default: 0.02 if -r, else 0.01)

-c CHROM, --circular=CHROM

specifies that chromosome CHROM is circular (default: chrM)

-V, --version

show program's version number and exit

Releases

7 releases since Debian 7 (Wheezy); the text changed 2 times.

  1. 7
  2. 8
  3. 9–13

--estdist since Debian 8 (Jessie); --version since Debian 9 (Stretch); -V since Debian 9 (Stretch); -e since Debian 8 (Jessie).

--genome until Debian 7 (Wheezy); -g until Debian 7 (Wheezy).

From last-align 1609-1+b1 in Debian 13 (Trixie). Parsed by carta from the page's man source.