wham1
Wisconsin's high-throughput alignment method
Overview
Usage:
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wham [options]* {<readfile> |
-1<m1file>-2<m2file>} <basepath> <output> <readfile> comma-separated list of files containing unpaired reads <m1file> comma-separated list of files containing upstream mates <m2file> comma-separated list of files containing downstream mates <basepath> write wham data to files with this dir/basename <output> file to write alignments to
Input options:
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-l <int> -
use first <int> bases in each read
Alignment options:
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-v <int> -
specify the max number of errors in a reported alignment.
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specify the max number of gaps in a reported alignment. |
|---|---|
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max sum of mismatch quals across alignment |
|
do not align to forward/reverse-complement ref strand |
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do not align to mate1/mate2 strand: fw/rev, rev/fw. |
|
minimum insert size for paired-end alignment (default: 0). |
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maximum insert size for paired-end alignment (default: 250). |
Reporting options:
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-k <int> -
report up to <int> valid alignemtns per read (default: 1).
-a/--all-
report all valid alignments per read.
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--best -
reprot valid alignments in a sorted order of quality.
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-m <int> -
discard reads with more than <int> valid alignmetns.
Output options:
-S/--sam-
write alignment in SAM format
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--al <fname> -
write aligned reads/pairs to file(s) <fname>
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--un <fname> -
write unaligned reads/pairs to file(s) <fname>
Performance options:
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-t <int> -
specify the number of threads
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--nocat -
do not concatenate results from various threads
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--step <int> -
specify the number of indexes that fit into memory.
Other options:
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--version -
print version information
-h/--help-
print this usage message
Releases
Unchanged in 3 releases since Debian 11 (Bullseye).