wham(1)

wham1

Wisconsin's high-throughput alignment method

Overview

Usage:

wham [options]* {<readfile> | -1 <m1file> -2 <m2file>} <basepath> <output> <readfile> comma-separated list of files containing unpaired reads <m1file> comma-separated list of files containing upstream mates <m2file> comma-separated list of files containing downstream mates <basepath> write wham data to files with this dir/basename <output> file to write alignments to

Input options:

-l <int>

use first <int> bases in each read

Alignment options:

-v <int>

specify the max number of errors in a reported alignment.

-g/--gap <int>

specify the max number of gaps in a reported alignment.

-e/--maqerr <int>

max sum of mismatch quals across alignment

--nofw/--norc

do not align to forward/reverse-complement ref strand

--nofr/--norf

do not align to mate1/mate2 strand: fw/rev, rev/fw.

-I/--minins <int>

minimum insert size for paired-end alignment (default: 0).

-X/--maxins <int>

maximum insert size for paired-end alignment (default: 250).

Reporting options:

-k <int>

report up to <int> valid alignemtns per read (default: 1).

-a/--all

report all valid alignments per read.

--best

reprot valid alignments in a sorted order of quality.

-m <int>

discard reads with more than <int> valid alignmetns.

Output options:

-S/--sam

write alignment in SAM format

--al <fname>

write aligned reads/pairs to file(s) <fname>

--un <fname>

write unaligned reads/pairs to file(s) <fname>

Performance options:

-t <int>

specify the number of threads

--nocat

do not concatenate results from various threads

--step <int>

specify the number of indexes that fit into memory.

Other options:

--version

print version information

-h/--help

print this usage message

Releases

Unchanged in 3 releases since Debian 11 (Bullseye).

  1. 11–13

Author

This manpage was written by Nilesh Patra for the Debian distribution and
can be used for any other usage of the program.

From wham-align 0.1.5-8 in Debian 13 (Trixie). Parsed by carta from the page's man source.