sand_filter_master1

Usage

Overview

sand_filter_master is the first step in the SAND assembler. It reads in a body of sequences, and uses a linear-time algorithm to produce a list of candidate sequences to be aligned in detail by sand_align_master(1).

This program uses the Work Queue system to distributed tasks among processors. After starting sand_filter_master, you must start a number of work_queue_worker(1) processes on remote machines. The workers will then connect back to the master process and begin executing tasks. The actual filtering is performed by sand_filter_kernel(1) on each machine.

Options

-p <port>

Port number for queue master to listen on. (default: 9123)

-s <size>

Number of sequences in each filtering task. (default: 1000)

-r <file>

A meryl file of repeat mers to be filtered out.

-R <n>

Automatically retry failed jobs up to n times. (default: 100)

-k <size>

The k-mer size to use in candidate selection (default is 22).

-w <size>

The minimizer window size. (default is 22).

-u

If set, do not unlink temporary binary output files.

-c <file>

Checkpoint filename; will be created if necessary.

-d <flag>

Enable debugging for this subsystem. (Try -d all to start.)

-F <number>

Work Queue fast abort multiplier. (default is 10.)

-Z <file>

Select port at random and write it out to this file.

-o <file>

Send debugging to this file.

-v

Show version string

-h

Show this help screen

Exit status

On success, returns zero. On failure, returns non-zero.

Examples

If you begin with a FASTA formatted file of reads, used sand_compress_reads(1) to produce a compressed FASTA (cfa) file. To run filtering sequentially, start a single work_queue_worker(1) process in the background. Then, invoke sand_filter_master.

% sand_compress_reads mydata.fasta mydata.cfa % work_queue_worker localhost 9123 & % sand_filter_master mydata.cfa mydata.cand

To speed up the process, run more work_queue_worker(1) processes on other machines, or use condor_submit_workers(1) or uge_submit_workers(1) to start hundreds of workers in your local batch system.