obijoinpairedend1
Description of obijoinpairedend obijoinpairedend aims at joining the two reads of a paired-end library. for this purpose, it concatenates sequence merging the forward read and the reversed-complemented reverse read. the program uses as input one or two sequences reads files.
Options
-
-r <FILENAME>, --reverse-reads=<FILENAME> -
Filename points to the file containing the reverse reads.
Options to specify input format
Restrict the analysis to a sub-part of the input file
- --skip <N>
-
The N first sequence records of the file are discarded from the analysis and not reported to the output file
- --only <N>
-
Only the N next sequence records of the file are analyzed. The following sequences in the file are neither analyzed, neither reported to the output file. This option can be used conjointly with the –skip option.
Sequence annotated format
--genbank-
Input file is in genbank format.
--embl-
Input file is in embl format.
fasta related format
--fasta-
Input file is in fasta format (including OBITools fasta extensions).
fastq related format
--sanger-
Input file is in Sanger fastq format (standard fastq used by HiSeq/MiSeq sequencers).
--solexa-
Input file is in fastq format produced by Solexa (Ga IIx) sequencers.
ecoPCR related format
--ecopcr-
Input file is in ecoPCR format.
--ecopcrdb-
Input is an ecoPCR database.
Specifying the sequence type
--nuc-
Input file contains nucleic sequences.
--prot-
Input file contains protein sequences.
Options to specify output format
Standard output format
--fasta-output-
Output sequences in
OBIToolsfasta format
--fastq-output-
Output sequences in Sanger fastq format
Generating an ecoPCR database
--ecopcrdb-output=<PREFIX_FILENAME>-
Creates an ecoPCR database from sequence records results
Miscellaneous option
--uppercase-
Print sequences in upper case (default is lower case)
Options
-
-h, --help -
Shows this help message and exits.
-
--DEBUG -
Sets logging in debug mode.