Selects primers for pcr and dna amplification.
prima -sequence sequence -targetrange toggle -targetstart integer -targetend integer -mintmprimer float -maxtmprimer float -minplen integer -maxplen integer -minpgccont float -maxpgccont float [-primer toggle] -minprimerlen integer -maxprimerlen integer -minpmgccont float -maxpmgccont float -overlap integer -saltconc float -dnaconc float -list boolean -outfile outfile prima -help
prima is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the "Nucleic:Primers" command group(s).
-sequence sequence
-targetrange toggle
Default value: N
-targetstart integer
Default value: $(sequence.begin)
-targetend integer
Default value: $(sequence.length)
-mintmprimer float
Default value: 53
-maxtmprimer float
Default value: 58
-minplen integer
Default value: 100
-maxplen integer
Default value: 300
-minpgccont float
Default value: .40
-maxpgccont float
Default value: .55
-primer toggle
Specify primer properties Default value: Y
-minprimerlen integer
Default value: 18
-maxprimerlen integer
Default value: 22
-minpmgccont float
Default value: .40
-maxpmgccont float
Default value: .55
-overlap integer
Default value: 50
-saltconc float
Default value: 50
-dnaconc float
Default value: 50
-list boolean
Default value: N
-outfile outfile
Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).
prima is fully documented via the tfm(1) system.
Debian Med Packaging Team <[email protected]>
Wrote the script used to autogenerate this manual page.
This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be redistributed under the same terms as EMBOSS itself.