Compare and report features of two similar sequences
diffseq -asequence sequence -bsequence sequence -wordsize integer [-globaldifferences boolean] -outfile report -aoutfeat featout -boutfeat featout diffseq -help
diffseq is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the "Alignment:Differences" command group(s).
-asequence sequence
-bsequence sequence
-wordsize integer
The similar regions between the two sequences are found by creating a hash table of 'wordsize'd subsequences. 10 is a reasonable default. Making this value larger (20?) may speed up the program slightly, but will mean that any two differences within 'wordsize' of each other will be grouped as a single region of difference. This value may be made smaller (4?) to improve the resolution of nearby differences, but the program will go much slower. Default value: 10
-globaldifferences boolean
Normally this program will find regions of identity that are the length of the specified word-size or greater and will then report the regions of difference between these matching regions. This works well and is what most people want if they are working with long overlapping nucleic acid sequences. You are usually not interested in the non-overlapping ends of these sequences. If you have protein sequences or short RNA sequences however, you will be interested in differences at the very ends . It this option is set to be true then the differences at the ends will also be reported. Default value: N
-outfile report
-aoutfeat featout
File for output of first sequence's features Default value: $(asequence.name).diffgff
-boutfeat featout
File for output of second sequence's features Default value: $(bsequence.name).diffgff
Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).
diffseq is fully documented via the tfm(1) system.
Debian Med Packaging Team <[email protected]>
Wrote the script used to autogenerate this manual page.
This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be redistributed under the same terms as EMBOSS itself.